Interactive notebooks hosted on nordinlab.org
Brønsted-Lowry acids and bases, conjugate pairs, and fundamental pH concepts for engineering students
Interactive exploration of buffer titrations, including Henderson-Hasselbalch equation and real-world engineering applications
Self-regulating pH titrations with weak bases — relevant to bioprocess and pharmaceutical applications
Analysis guide and experimental/processing plan for Allura Red on-chip urea assay imaging.
Allura Red channel height estimation and sensitivity analysis across analysis stages 5-8.
Adsorption diagnostic (Stage 4a) checking for Allura Red adsorption in a BSA-passivated channel.
Allura Red x 510/10 filter polychromatic Beer-Lambert analysis using measured Nanodrop spectra.
Allura Red absorbance analysis with 510/10 nm narrow-bandpass filter for the on-chip urea assay.
WASM marimo app analysing Keyence microscopy data for the urea assay (read-only / run mode).
Spectral analysis of dye absorbance (Phenol Red, BPB) vs filter transmission for the on-chip urea assay.
Idealized model of BPB absorption vs TRITC bandpass quantifying Jensen's-inequality polychromatic deviation from Beer-Lambert
Beer–Lambert calibration and ROI analysis of Bromophenol Blue in a capillary slide.
Bromophenol Blue transmittance and absorbance analysis with calibration curves and nominal vs actual channel height comparison.
Bromophenol Blue absorbance spectrum overlaid with TRITC filter transmission and effective absorbance calculation.
Measures microfluidic channel heights using Bromophenol Blue absorbance and Beer–Lambert linearity validation.
Analyses green-channel drift and variability in flat-field images across timepoints and channel sections.
Characterises light source stability and within-burst noise for TRITC urea assay imaging across timepoints and channel sections.
Blank-subtracted Beer–Lambert calibration using ΔA vs path length. Unknowns predicted with 1–11% error (excl. 0.75 mM).
Calibration and unknown concentration prediction from same-chip TRITC data — 5 unknowns validated with 5–13% error.
Validates calibration transfer to unknown urea samples — predicts concentrations from absorbance images using 8-channel fits
Compares single-frame vs 30-frame averaging for absorbance calibration — slope differences are ≤0.3%, so a single corrected frame suffices.
Colorimetric assay with calibration concentrations in multiple-height channel
UV-Vis spectrophotometry analysis of urea assay with calibration curves and spectral visualization